Do You Know HAV Test Strips/Boxes?

Let's follow the Virus Sample Collection Kit Supplier to learn about the HAV Test Cassette or HAV Test Strip.

Common Name: The introduction of diagnostic kit(colloidal gold technique) for IgM of hepatitis A and E

English Name: Diagnostic kit for anti-HAV-HEV IgM (Colloidal Gold)

Intended Use:

It is used for diagnostic of anti-HAV-HEV IgM from human serum(or plasma) and can be used clinically.

Hepatitis A is an acute transmitted disease infected by hepatitis A virus(HAV) which belongs topicornaviridae, hepatovirus. It is transmitted through faecal-oral route and commonly seen in children and teenagers. Hepatitis A is self-limiting and rarely seen as chronic infection. There are 7 gene types of hepatitis A but there is only one serotype of HAV. So there is only one antigen-antibody system, which is antibody IgM and igG. IgM appears earlier and can be lasted for 8-12 weeks and in a few cases it can be lasted for 6 months. IgG can be lasted several years and is mainly protective antibody. Hepatitis A can be transmitted in three ways:(1)Daily life contact;(2)Aquagenic transmission;(3)Food. Otherwise, fly and cockroach is the main vector of the disease.

Hepatitis E(HE) is an acute transmitted disease caused by HEV and infected mainly through water and also food and daily life. It is formerly called enterically transmitted non-A, non-B hepatitis, leading to eruption or epidemic, and also sometimes sporadic. The mortality of HEV is about 0.5%-1.5%, a little higher than that of HAV. But the mortality of HEV in pregnant women can reach to 20%.China is among the high prevalence country in the world and take up 10%-20% of all cases with hepatitis. There are 4 gene types of HEV and type 1 gene is most commonly seen in developing countries, such as China and some African countries. Type 2 gene is found in Mexico and some African countries; Type 3 gene is discovered over the world and can be tested in many cases with acute hepatitis which are out of Africa; Type 4 gene is found only is Asian. Type 1 gene is with highest prevalence in our country and type 4 gene can also be tested. There is so far one serotype of HEV.

Diagnostic Principles:

HAV Test Strip

The anti-HAV-IgM kit depends on the capture method and gold immunochromatography assay. The HAV antigen and mouse monoclonal antibody are coating in polyester fibre(or non-woven fibrics) and the test lines with nitrocellulose membrane and control lines are coated with human IgMµ chain monoclonal antibody and IgG antibody separately. When the positive samples are added, the IgM antibody of HAV virus in the serum of plasma can combined with the HAV-antigen in the colloidal gold. The compound can move forward due to the chromatography effect and form ‘Au-HAV Ag-HAV anti-human IgM Ab’ with pre-coated anti-human IgMµ chain monoclonal antibody when passing the testing lines. As a result, the lines turn to be amaranth. In the control lines, the free gold standard mouse monoclonal antibody can attach to the anti-mouse IgG antibody and make the control lines amaranth. But in the negative group, the amaranth is only found in control lines.

The anti-HEV-IgM kit depends on capture method and gold immunochromatography assay. The HEV antigen and mouse monoclonal antibody are coating in polyester fibre(or non-woven fibrics) and the test lines with nitrocellulose membrane and control lines are coated with human IgMµ chain monoclonal antibody and IgG antibody separately. When the positive samples are added, the IgM antibody of HEV virus in the serum of plasma can combined with the HAV-antigen in the colloidal gold. The compound can move forward due to the chromatography effect and form ‘Au-HEV Ag-HEV anti-human IgM Ab’ with pre-coated anti-human IgMµ chain monoclonal antibody when passing the testing lines. As a result, the lines turn to be amaranth. In the control lines, the free gold standard mouse monoclonal antibody can attach to the anti-mouse IgG antibody and make the control lines amaranth. But in the negative group, the amaranth is only found in control lines.